Технологические решения для строительства разведочной вертикальной скважины глубиной 2620 метров на нефтяном месторождении (Томская область)

Объектом исследования является разведочная вертикальная скважина глубиной 2620 метров. Целью работы подразумевает – проектирование технологического решения для бурения вертикальной разведочной скважины глубиной 2620 метров на месторождении Томской области. Для достижения поставленной цели были поставлены следующие задачи: 1.Спроектировать конструкцию скважины. 2.Спроектировать процессы углубления скважины. 3.Спроектировать процессы заканчивания скважин. 4.Провести анализ цементных составов для многолетнемерзлых пород при строительстве нефтяных и газовых скважин. 5.Составить нормативную карту строительства и произвести расчёт сметной стоимости бурения и крепления скважины. 6.Произвести анализ производственной и экологической безопасности, а также безопасности при чрезвычайных ситуациях.The object of research is an exploration vertical well with a depth of 2620 meters. The purpose of the project is to design a technological solution for drilling a vertical exploration well with a depth of 2620 meters in the field of the Tomsk region. To achieve this goal, the following tasks were set: 1. Design the well structure. 2. Design the processes of well deepening. 3. Design well completion processes. 4. analyze cement compositions for permafrost during the construction of oil and gas wells. 5. Draw up a standard construction map and calculate the estimated cost of drilling and fixing the well. 6. analyze industrial and environmental safety, as well as safety in emergency situations

Supplementary data for article: Yu, E. H.; Prodanović, R.; Gueven, G.; Ostafe, R.; Schwaneberg, U. Electrochemical Oxidation of Glucose Using Mutant Glucose Oxidase from Directed Protein Evolution for Biosensor and Biofuel Cell Applications. Applied Biochemistry and Biotechnology 2011, 165 (7–8), 1448–1457. https://doi.org/10.1007/s12010-011-9366-0

Supplementary material for: [https://doi.org/10.1007/s12010-011-9366-0]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1221

Supplementary data for article: Yu, E. H.; Prodanović, R.; Gueven, G.; Ostafe, R.; Schwaneberg, U. Electrochemical Oxidation of Glucose Using Mutant Glucose Oxidase from Directed Protein Evolution for Biosensor and Biofuel Cell Applications. Applied Biochemistry and Biotechnology 2011, 165 (7–8), 1448–1457. https://doi.org/10.1007/s12010-011-9366-0

Supplementary material for: [https://doi.org/10.1007/s12010-011-9366-0]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1221

Electrochemical Oxidation of Glucose Using Mutant Glucose Oxidase from Directed Protein Evolution for Biosensor and Biofuel Cell Applications

In this study, electrochemical characterisation of glucose oxidation has been carried out in solution and using enzyme polymer electrodes prepared by mutant glucose oxidase (B11-GOx) obtained from directed protein evolution and wild-type enzymes. Higher glucose oxidation currents were obtained from B11-GOx both in solution and polymer electrodes compared to wt-GOx. This demonstrates an improved electrocatalytic activity towards electrochemical oxidation of glucose from the mutant enzyme. The enzyme electrode with B11-GOx also showed a faster electron transfer indicating a better electronic interaction with the polymer mediator. These encouraging results have shown a promising application of enzymes developed by directed evolution tailored for the applications of biosensors and biofuel cells.Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3611